TOP and best 80 question with answer of Protein and metabolism MCQ ,D.Pharm,B.Pharm BIOCHEMISTRY, 12 CLASS, BIOLOGY,AND COMPITATIVE, EXAM,NEET ,POLY TECHNIQUE,OTHER EXAM

1.All proteins contain the
(A ) Same 20 amino acids
(B )Different amino acids
(C )300 Amino acids occurring in nature
( D)Only a few amino acids
2.Proteins contain
(A) Only L- Alpha- amino acids
(B ) Only D-amino acids
(C ) DL-Amino acids
(D )Both (A) and (B)
3.Sulphur containing amino acid is
   (A ) Methionine
   (B) Leucine
(C) Valine
(D) Asparagine
4.All the following are sulphur containing amino acids found in proteins except
    (A ) Cysteine
   (B) Cystine
(C) Methionine
(D) Threonine
5.An aromatic amino acid is
    (A ) Lysine
   (B) Tyrosine
(C) Taurine
(D) Arginine
6.The functions of plasma albumin are
(A )Osmosis
(B) Transport
(C) Immunity
(D) both (A )and (B)
7.An essential amino acid in man is
(A )Aspartate
(B) Tyrosine
(C) Methionine
(D) Serine
8.Non essential amino acids
(A ) Are not components of tissue proteins
(B ) May be synthesized in the body from essential amino acids
(C ) Have no role in the metabolism
(D ) May be synthesized in the body in diseased states
9.Which one of the following is semi- essential amino acid for humans?
(A )Valine
(B) Arginine
(C) Lysine
(D) Tyrosine
10.Biuret reaction is specific for
(A )–CONH-linkages
(B) –CSNH2 group
(C) –(NH)NH₂ group
(D) All of these
11.Denaturation of proteins results in
(A ) Disruption of primary structure
(B ) Breakdown of peptide bonds
(C ) Destruction of hydrogen bonds
(D ) Irreversible changes in the molecule
12. A ’suicide enzyme’ is
(A ) Cycloxygenase
(B) Lipooxygenase
(C) Phospholipase A₁
(D) Phospholipase

13. A positive nitrogen balance occurs
(A ) In growing infant
(B ) Following surgery
(C ) In advanced cancer
(D ) In kwashiorkar
14. A compound serving a link between citric acid cycle and urea cycle is
(A ) Malate
(B) Citrate
(C) Succinate
(D)Fumarate

15. In humans the sulphur of methionine and cysteine is excreted mainly as
(A ) Ethereal sulphate
(B ) Inorganic sulphate
(C ) Sulphites
(D ) Thioorganic compound
16. The amino acids involved in the synthesis of creatin are
(A ) Arginine, glycine, active methionine
(B ) Arginine, alanine, glycine
(C ) Glycine, lysine, methionine
(D ) Arginine, lysine, methionine
17. A conjugated protein present in the egg yolk is
(A )Vitellin
(B) Livetin
(C) Albuminoids
(D) Ovo-mucoid
18. The chief protein of cow’s milk is
(A ) Albumin
(B) Vitellin
(C) Livetin
(D) Casein

19. Pulses are rich in
(A ) Lysine
(B) Methionine
(C) Tryptophan
(D) Phenylalanine
20. The basic amino acids are
(A ) Lysine
(B) Bile acids
(C) Glycine
(D) Alanine
21. A coagulated protein is
(A ) Insoluble
(B ) Biologically non-functional
(C ) Unfolded
(D ) All of the above

22. Primary structure of proteins can be determined by the use of
(A )Electrophoresis
(B) Chromatography
(C) Ninhydrin
(D) Sanger’s reagent
23. The most abundant protein in mammals is
(A )Albumin
(B) Haemoglobin
(C) Collagen
(D) Elastin
24. Primary structure of a protein is formed by
(A )Hydrogen bonds
(B) Peptide bonds
(C) Disulphide bonds
(D) All of these
25. Aromatic amino acids can be detected by
(A ) Sakaguchi reaction
(B ) Millon-Nasse reaction
(C ) Hopkins-Cole reaction
(D ) Xanthoproteic reaction
26. All the following are branched chain amino acids except
(A ) Isoleucine
(B) Alanine
(C) Leucine
(D) Valine
27. An –OH group is present in the side chain of
(A )Serine
(B) Arginine
(C) Lysine
(D) Proline

28. An organ which is extremely sensitive to ammonia toxicity is
(A )Liver
(B) Brain
(C) Kidney
(D) Heart
29. The major site of urea synthesis is
(A )Brain
(B) Kidneys
(C) Liver
(D) Muscles

30. Histamine is formed from histidine by
(A )Deamination
(B) Dehydrogenation
(C) Decarboxylation
(D) Carboxylation
31. A Zwitterion is
(A )Positive ion
(B) Negative ion
(C) Both (A) and (C)
(D) None of these

32. Biological value of a protein is
(A ) The percentage of ingested protein/nitrogen absorbed into circulation
(B ) The percentage of ingested protein/nitrogen in the body
(C ) The percentage of ingested protein utilised for protein synthesis in the body
(D )The gain in body weight (gm) per gm of protein ingested

33. For vegetarians, pulses are an important source of
(A )Carbohydrates
(B) Proteins
(C) Fat
(D) Iron
34. Milk is a good source of
(A ) Proteins, calcium and iron
(B ) Proteins, calcium and ascorbic acid
(C ) Proteins, lactose and retinol
(D ) Proteins, lactose and essential fatty acids

35. Cholesterol is present in all the following except
(A )Milk
(B) Fish
(C) Egg white
(D) Egg yolk
36. Kwashiorkor occurs when the diet is severely deficient in
(A )Iron
(B) Calories
(C) Proteins
(D) Essential fatty acids
37. Kwashiorkor usually occurs in
(A )The post-weaning period
(B )Pregnancy
(D )Lactation
(E )Old age
38. Marasmus occurs from deficient intake of
(A )Essential amino acids
(B )Essential fatty acids
(C ) Calories
(D ) Zinc
39. Marasmus differs from Kwashiorkor in the which of these following respect
(A ) Mental retardation occurs in kwashiorkor but not in marasmus
( B) Growth is retarded in kwashiorkor but not in marasmus
(C ) Muscle wasting occurs in marasmus but not kwashiorkor
(D ) Subcutaneous fat disappears in marasmus but not in kwashiorkor

40. Sakaguchi reaction is answered by
(A ) Lysine
(B ) Ornithine
(C ) Arginine
(D ) Arginino succinic acid
41. The amino acid exist as Zwitter ions when they are in
(A )solid state
(B) acidic solution
(C) alkaline solution
(D) neutral solution
42. Plasma proteins are isolated by
(A ) Salting out
(B) Electrophoresis
(C) Flourimetry
(D) Both (A) and (B)

43. An amino acid not involved in urea cycle is
(A )Arginine
(B) Histidine
(C) Ornithine
(D) Citrulline
44. For biosynthesis of proteins
(A ) Amino acids only are required
(B ) Amino acids and nucleic acids only are required
(C ) Amino acid, nucleic acids and ATP only are required
(D ) Amino acids, nucleic acids, ATP, GTP, enzymes and activators are required

45. The milk protein in the stomach of the infants is digested by
(A )Pepsin
(B) Trypsin
(C) Chymotrypsin
(D) Rennin
46.    The pH of gastric juice become low in
(A )Hemolytic anemia
(B) Pernicious anemia
(C) Both (A) and (B)
(D) None of these
47.  Chymotrypsin in the small intestine hydrolyzes peptide linkages containing
(A )Alanine
(B) Pheynl alanine
(C) Valine
(D) Methionine
48.    If one amino acid is fed excess, the absorption of another is
(A ) Slightly accelerated
(B ) Moderately accelerated
(C ) Highly accelerated
(D ) Retarded
49.    Transamination is a
(A )Irreversible process
(B) Reversible process
(C) Both (A) and (B)
(D) None of these
50. Ammonia production by the kidney is depressed in
(A ) Acidosis
(B) Alkalosis
(C) Both (A) and (B)
(D) None of these
51. The biosynthesis of Urea occurs mainly in the Liver:
(A ) Cytosol
(B ) Microsomes
(C ) Nucleus
(D ) Mitochondria
52. Clinical symptom in urea cycle disorder is
(A) Mental retardation
(B) Drowsiness
(C) Diarrhoea
(D) Oedema
53. Plasma proteins act as
(A)Buffers
(B) Immunoglobulins
(C) Reserve proteins
(D) All of these
54. Group that reacts in the Biuret test:
(A )Peptide
(B) Amino group
(C) Carboxylic group
(D) Aldehyde group
55. Protein present in hemoglobin has the structure known as
(A )Primary
(B) Secondary
(C) Tertiary
(D) Quarternary
56. Optical isomers of all aminoacids exist except
(A ) Glycine
(B) Arginine
(C) Alanine
(D) Hydroxy proline
57. Side chains of all following amino acids contain aromatic rings except
(A ) Phenyl alanine
(B) Alanine
(C) Tyrosine
(D) Tryptophan
58. Insulin is made up of
(A ) A single polypeptide chain having 51 amino acid residues
(B ) A single polypeptide chain having 84 amino acid residues
(C ) A-chain having 21 and B-chain having 30 amino acid residues
(D ) A-chain having 30 and B-chain having 21 amino acid residues
59. Immunoglobulins are classified on the basis of
(A ) Type of light chains
(B ) Type of heavy chains
(C ) Types of light and heavy chains
(D ) Molecular weight
60. In denaturation of proteins, the bond which is not broken:
(A )Disulphide bond
(B) Peptide bond
(C) Hydrogen bond
(D) Ionic bond

61. Protein is a polymer of
(A )Sugars
(B) Phenols
(C) Amino acids
(D) Carboxylic acids
62. Insulin is a protein which controls
( A)Blood clotting
(B) Metabolic pathway
(C) Digestion
(D) Kreb’s cycle
63. Proteins which are responsible for defence mechanism are called
(A ) Antimetabolites
(B) Antibodies
(C) Antimycins
(D) Apoproteins
64. Isoelectric point of amino acids is used for
(A ) Crystallisation
(B) Precipitation
(C) Solubility
(D) Reactivity
65. Million’s test is positive for
(A )Phenylalanine
(B) Glycine
(C) Tyrosine
(D) Proline
66. Guanidine group of argentine gives positive test with
(A ) Lead acetate
(B ) Sakaguchi reagent
(C ) Tricholoroacetic acid
(D ) Molisch’s reagent
67. Thiol group of cysteine gives red colour with
(A ) Sodium acetate
(B ) Lead acetate
(C ) Sodium nitroprusside
(D )Barfoed’s reagent
125.Protein deficiency disease is known as
(A )Cushing’s disease
(B )Fabry’s disease
(C )Parkinson’s disease
( D ) Kwashiorkor and marasmus
68. A vegetable source of protein is
(A ) Egg plant
(B ) Soyabean
(C ) Tree of the Heaven
(D ) Devil’s dung
69. Oxaloacetate is converted to aspartic acid by
(A)  Reductase
(B)  Oxidase
(C) Transminase
(D) Catalase
70.Biuret test is specific for
(A ) Two peptide linkage
(B ) Phenolic group
(C ) Imidazole ring
(D ) None of these
71. During glycolysis of glucose the energy liberated in the absence of oxygen is known as
(A)     Oxygenesis
(B)     Glyconeogenesis
(C )    Glycogenolysis
  (D)   Anaerobic fermentation
72. Accumulation of trytophan in blood is known as
(A)Pompe’s disease
(B) Wilson’s disease
(C) Wolman’s disease
(D) Hartnup’s disease
73. Lymphocytes are responsible for the for- mation of
(A )Serum
(B) Plasma
(C) Antibody
(D) Calcium

74. All the following are true about phenyl- ketonuria except
(A)     Deficiency of phenylalanine hydroxylase
(B)     Mental retardation
          (C ) Increased urinary excretion of p-hydroxy- phenyl pyruvic acid
(D ) Decrease serotonin formation
75. Neutral amino acid is
(A)Leucine
(B) Lysine
(C) Aspartic acid
(D) Histidine
76.The amino acid containing hydroxy group:
(A)Glycine
(B) Isoleucine
(C) Arginine
(D) Thereonine

77. The milk protein in the stomach of the infants is digested by
(A) Pepsin
           (B) Trypsin
(C) Chymotrypsin
(D) Rennin
78. Sakaguchi reaction is specific for
(A)     Guanidine group
(B) Phenolic group
(C) Carboxylic group
(D) None of these
79. With the exception of glycine all amino acids found in protein are
(A) Isocitrate dehydrogenase
(B) Fumarase
(C) Succinate thiokinase
(D )ATPase

80. The protein present in hair is
(A)Elastin
(B) Prolamine

(D) Gliadin