Plant cell, tissue culture ,and its application -mcq questions and answers

 

 

1.Plant biotechnology involves
a)   production of valuable products in plants      
b)  rapid clonal multiplication of desired genotypes
c)  production of virus free plants                     
d)  all of these
 
2.The most common solidifying agent used in micropropagation is
a)  agar                           
b)  dextran                      
c)  Mannan                     
d)  all of these
 
3.The culturing of cells in liquid agitated medium is called
a)  liquid culture          
b)  micro propagation  
c)  Agar culture           
d)  suspension culture
 
4.Which of the following is best suited method for production of virus free plants
a)  embryo culture            
b)  meristem culture         
c)  ovule culture               
d)  anther culture
 
5.Batch cultures are type of suspension culture where
a)  medium is continuously replaced                          
b)  medium is loaded only at the beginning
c)  no depletion of medium occurs        
d)  cellular wastes are continuously removed and replaced
 
6. All are plant derived alkaloids except
a)  menthol               
b)  nicotine               
c)  quinine                
d)  codeine
 
7. Elicitors are molecules that
   a)  induce cell divison                                      
b)  stimulate production secondary metabolites
c)  stimulate hairy root formation that accumulate secondary metabolites     
d)none of these
 
8. All are plant derived elicitors except
a)  chitin                     
b)  pectin                    
c)  cellulose                 
d)  pectic acid

9.     The modification of exogenous compounds by plant cells is called
a)  Biotransformation        
b)  bioconversion               
c)  both a and b                 
d)  biophytomodification
 
10. Artificial seeds are
a) seeds produced in laboratory condition                                
b) seeds encapsulated in a a gel
c) somatic embryos encapsulated in a gel                     
d) zygotic embryos encapsulated in a gel

11.  Hairy root cultures for secondary metabolite production are induced by transforming plant cells withs
a) virus                                                                           
b) Agrobacterium tumefaciens
c) Bacillzus thuringiensis                                                 
d) Agrobacterium rhizogenes
 
12. The variation in invitro culture is called as
a) invitro variation            
b) mutation                       
c) somaclonal variation     
d) all of these
 
13. Haploid plants are produced in large numbers by
a) anther culture             
b) Ovary culture             
c) both a and b               
d) embryo culture
 
 
14. In plant tissue culture, what is term ORGANOGENESIS mean ?
a) formation of callus culture                    
b) formation of root and shoot from callus culture
c) genesis of plants                                 
d) none of the above
 
15. A plant called Rauvolfia serpentina is under the threat of extinction. To save this plant, which technique is highly useful?
a). genetic engineering                              
b). DNA finger printing
c). hybridoma technology                          
d). in vitro culture
 
16. A device in which a substrate of low value is utilized by living cells or enzymes to generate a product of higher value is called
a). bioreactor             
b). test tube culture   
c). Electrophoresis     
d). chromotography
 
17. A bioreactor known for mass culturing of cells / microbes must have
a). agitation for mixing of cells and medium                
b). sterile conditions
c). regulation of temperature, aeration, etc.,                 
d). all of these
 
18. Bioreactors are used for
   a) large scale production of desired substances by using cells /microbes
   b) kill bacteria                        
   c) to store viruses                   
   d) to get chemicals
 
19. The basic components of tissue culture media are
a)  micro and macro nutrients, glucose
b)  micro and macro nutrients, vitamins, agar
c)  micro and macro nutrients and growth regulators, glucose
d)  micro and macro nutrients, growth regulators, agar, vitamins, glucose
 
 20. Agar agar is added to tissue culture media as
a). carbon source                                                     
b). a growth regulator
c). nitrogen source                                                  
d). solidifying agent
 
21. Agar agar, used in plant tissue culture is extracted from,
a). a fungi           
b). a bacteria       
c). an algae         
d). a virus
 
22. Glucose is added to the tissue culture media as
a). growth regulator                                                   
b). carbon source
c). solidifying agent                                                   
d). an antibiotic
 
23. Explant is
a)  any cut part of the plant used in tissue culture
b)  a plant extract used in tissue culture
 c)   a source of growth regulators added to media
d)   solidifying agent
 
24. Totipotency refers to
a) the ability of a plant cell to arrest the growth of a plant
b)  the ability of a plant cell to develop disease in plant
c)  the ability of a plant cell to develop into a complete plant
d) the inability of a plant cell to develop into a callus
 
 
25. In vitro culture of plant parts need
a). controlled environmental condition                                   
  b). aseptic condition
  c). maintenance of pH                                                         
d). all of these
 
26. An amorphous mass of loosely arranged thin walled parenchyma cells developing from explant is called
 
a). thallus                          
b). Callus                          
c). Callose                        
d). embryoids
 
27. The unique feature of callus is
a). it gives rise to cells only                                              
b). it can give rise to zygotic embryos
c). it can give rise to root, shoot and embryoids                  
d). it can give rise to flowers directly
 
28. Meristem culture helps in developing
a). hybrid plants                                                                            
b). virus free plants
c). disease resistant plants                                                              
d). tall plants
 
29. Genetic variation observed in callus obtained from tissue culture is called
a). morphogenesis                                                                          
b). rhizogenesis
c). callogenesis                                                                              
d). somaclonal variation
 
30. Zinc as micronutrient is used in culture media for _____________
a) Protein synthesis
b) DNA replication
c) For plant tissue culture
d) For photosynthesis



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